Systems Approaches for Exploring and Exploiting Cellular Metabolism View Edit Track

Saturday, March 23, 2013 - 2:00pm
Fung Auditorium | Powell-Focht Bioengineering Hall
Jennifer Reed, PhD

Department of Chemical and Biological Engineering
Great Lakes Bioenergy Research Center
University of Wisconsin-Madision

Systems Approaches for Exploring and Exploiting Cellular Metabolism View Edit Track

Abstract: 

Genome-scale networks of metabolism and regulation can be reconstructed from
an organism’s genome annotation. Models generated from these reconstructions
can be used to integrate and analyze different types of experimental data in order
to generate hypotheses about biochemical network structure, interactions between
organisms and their environments, and responses to genetic and environmental
perturbations. Examples from modeling different bacteria with biotechnology and
metabolic engineering applications will be presented to illustrate how modeling
and experimental efforts can be combined to improve computational models and
our understanding of cellular metabolism and regulation. Once accurate models
are developed, computational tools can be applied to design microbial strains
with enhanced chemical production. We have recently developed new methods
for proposing metabolic engineering strategies based on metabolic and regulatory
model predictions. These strategies can involve transcription factor and metabolic
gene deletions, as well as enzyme over expression. Using these approaches we have
identified genetic strategies for improving production of a variety of biochemicals in
E. coli including ethanol, isobutanol, succinate, amino acids, and malate.